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By John H. Hash

The significantly acclaimed laboratory commonplace, equipment in Enzymology, is without doubt one of the such a lot hugely revered guides within the box of biochemistry. considering 1955, every one quantity has been eagerly awaited, usually consulted, and praised by means of researchers and reviewers alike. The sequence comprises a lot fabric nonetheless suitable this present day - really an important book for researchers in all fields of existence sciences.

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C. Mitomycin . . . . . . . . IV. Compatibility Properties of R Plasmids . . . . A. Determination of fi Character . . . . . B. Testing for Compatibility of R Factors . . . . . . . . . . . . . . 41 43 43 45 48 49 51 51 52 52 54 54 I. I n t r o d u c t i o n T h e extensive use of c h e m o t h e r a p e u t i c agents has p r o v i d e d a n e n v i r o n m e n t conducive to the selection of b a c t e r i a l s t r a i n s I r e s i s t a n t to these agents.

VI. Selection from Mutagen-Treated Populations . . . . . 24 25 26 26 27 29 31 32 35 36 36 I. I n t r o d u c t i o n Strain development, or strain improvement, is a key factor in the development of an antibiotic as a potential therapeutic agent. Before any satisfactory biological testing and chemical characterization of a new antibiotic entity can be accomplished, adequate amounts of the material obviously must be available. As a rule, however, it soon becomes apparent t h a t the producing culture is synthesizing totally inadequate amounts of the antibiotic; thus, the problem of increasing yields becomes one of primary importance, and a pressing one.

41 43 43 45 48 49 51 51 52 52 54 54 I. I n t r o d u c t i o n T h e extensive use of c h e m o t h e r a p e u t i c agents has p r o v i d e d a n e n v i r o n m e n t conducive to the selection of b a c t e r i a l s t r a i n s I r e s i s t a n t to these agents. Since t h e i r discovery in J a p a n in the 1950's, the occurrence of b a c t e r i a r e s i s t a n t to a n t i m i c r o b i a l agents in clinical s i t u a t i o n s has been reported t h r o u g h o u t the world, a n d it is n o w clear t h a t the presence of such r e s i s t a n t b a c t e r i a can, u n d e r m a n y circumstances, prove to be a serious i m p e d i m e n t to n o r m a l a n t i b a c t e r i a l t h e r a p y Y T h e m e c h a n i s m s of r e s i s t a n c e to m a n y of the c o m m o n l y used a n t i m i c r o b i a l agents have 1Unfortunately the bacterial strains and bacteriophage stocks referred to in this article "are not available from any central source.

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