By Kevin D. Altria (auth.)
During the 1980's the research of prescription drugs was once ruled by way of excessive Perfor mance Liquid Chromatography (HPLC). different separative concepts similar to fuel Chromato graphy and skinny Layer Chromatography provided choices yet their quantitative capabili ties and/or solute diversity couldn't method that of HPLC. nearly all of prescription drugs are ionic and it'd be average to imagine that electrophoresis will be beneficial within the research of prescription drugs. even if, the electrophore tic tools to be had within the 1980's have been labour extensive and hired post-separation detection techniques. through the overdue 1980's and early 1990's wide study used to be con ducted into the probabilities of accomplishing electrophoretic separations in capillaries. This strategy allowed online detection and will be played on absolutely automatic apparatus. This learn resulted in the arrival of recent day capillary electrophoresis (CE) tools which supply comparable functionality and automation degrees to that of HPLC. study was once additionally all for constructing purposes for CE and specific awareness was once paid to functions in the pharmaceutical research region. those functions proved that CE will be utilized to a variety of drug varieties together with water insoluble and impartial compounds. the facility to accomplish effective chiral separations of substances additionally elevated the recognition of the strategy. CE with oblique UV detection has turn into verified as an easy and powerful replacement to ion-exchange chromatography for the decision of small inorganic or natural ions.
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Additional info for Analysis of Pharmaceuticals by Capillary Electrophoresis
Quaglia MG, Farina P, Bossu E, and Dell' Aquila C, Analysis of non-benzodiapenic anxiolytic agents by capillary zone electrophoresis, J. Pharm. Biomed. , 13 (1995) 505-509. 10. Bjornstdottir I and Hansen SH, Determination of opium alkaloids in opium by capillary electrophoresis, J. Pharm. Biomed. , 13 (1995) 687-693. II. Altria KD and Chanter YL, Validation of a capillary electrophoresis method for the determination of a quinolone antibiotic and its related impurities, J. , 652 (1993) 459-463. 12.
A typical electrolyte composition (28, 34-36) for MECC may be 10 mM borate containing 40 mM SDS. Analysis of very water-insoluble drugs can be performed by addition of organic solvents to the MECC electrolyte. Levels of organic solvents such as methanol or acetonitrile may be used up to 30 % v/v to improve solute solubility. Alternatively (43, 44) lower levels such as 5-10 % of solvents such as propanol, acetone, DMSO or butan-I-ol can be employed. Other approaches to analysis of water-insoluble compounds using SDS based MECC, include use of SDS in combination with cyclodextrins, or with high levels of urea (45) which significantly improves solute solubility.
5. 5) has been extensively validated (14) as a general method for the analysis of a range of acidic drugs. 1 %). Validation showed acceptable linearity, 28 2 Main Component Assay by CE reagent stability (shelf-life of 3 months) and linearity. Borate has minimal UV background which permits use of low UV wavelengths to achieve acceptable sensitivities in the order of single figure mgIL values. 6). 3 shows a typical separation of an acidic drug (warfarin) and the two internal standards. Water-insoluble acidic compounds were dissolved in 10 mM NaOH.